Title

Drosophila Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution

Status

Faculty

School

School of Science

Department

Biology

Document Type

Article

Publication Date

11-2017

Publication Title

G3: GENES, GENOMES, GENETICS

Description/Abstract

The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it is mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution of repeats and genes, we manually improved the sequence and annotated the genes on the D. erecta, D. mojavensis, and D. grimshawi F elements and euchromatic domains from the Muller D element. We find that F elements have higher transposon density (25%–50%) than euchromatic reference regions (3%–11%). Among the F elements, D. grimshawi has the lowest transposon density (particularly DINE-1: 2% versus 11%–27%). F element genes have larger coding spans, more coding exons, larger introns, and lower codon bias. Comparison of the Effective Number of Codons with the Codon Adaptation Index shows that, in contrast to the other species, codon bias in D. grimshawi F element genes can be attributed primarily to selection instead of mutational biases, suggesting that density and types of transposons affect the degree of local heterochromatin formation. F element genes have lower estimated DNA melting temperatures than D element genes, potentially facilitating transcription through heterochromatin. Most F element genes (~90%) have remained on that element, but the F element has smaller syntenic blocks than genome averages (3.4-3.6 versus 8.4-8.8 genes per block), indicating higher rates of inversion despite lower rates of recombination. Overall, the F element has maintained characteristics that are distinct from other autosomes in the Drosophilalineage, illuminating the constraints imposed by a heterochromatic milieu.

Keywords

CODON BIAS, EVOLUTION OF HETEROCHROMATIN, GENE SIZE, MELTING CHARACTERISTICS, TRANSPOSONS

Volume

7

Issue

11

Scholarly

yes

DOI

10.1534/g3.114.015966

Disciplines

Biology | Genetics and Genomics

Comments

Article written with participating students and faculty of the Genomics Education Partnership including the following Saint Mary’s students: Christopher Beck, Kristen R. Hatfield, Douglas A. Herrick, Christopher B. Khoury, Charlotte Lea, Christopher A. Louie, Shannon M. Lowell, Thomas J. Reynolds, Jeanine Schibler, Alexandra H. Scoma, Maxwell T. Smith-Gee, Sarah Tubert.

Original Citation

Vidya Chandrasekaran (Biology): “Drosophila Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution,” with Wilson Leung (primary author) and Participating Students and Faulty of the Genomics Education Partnership, in G3 vol. 7 no. 11; Nov. 2017. https://doi.org/10.1534/g3.114.015966

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