Leukemia inhibitory factor and ciliary neurotrophic factor cause dendritic retraction in cultured sympathetic neurons

SMC Author

Vidya Chandrasekaran

SMC Affiliated Work

1

Author Role

co-author

Status

Faculty

School

School of Science

Department

Biology

Document Type

Article

Publication Date

3-15-1999

Publication / Conference / Sponsorship

J. Neurosci

Description/Abstract

Dendritic retraction occurs in many regions of the developing brain and also after neural injury. However, the molecules that regulate this important regressive process remain largely unknown. Our data indicate that leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) cause sympathetic neurons to retract their dendrites in vitro, ultimately leading to an ∼80% reduction in the size of the arbor. The dendritic retraction induced by LIF exhibited substantial specificity because it was not accompanied by changes in cell number, in the rate of axonal growth, or in the expression of axonal cytoskeletal elements. An antibody to gp130 blocked the effects of LIF and CNTF, and both cytokines induced phosphorylation and nuclear translocation of stat3. Moreover, addition of soluble interleukin-6 (IL-6) receptor to the medium endowed IL-6 with the ability to cause dendritic regression. These data indicate that ligands activating the gp130 pathway have the ability to profoundly alter neuronal cell shape and polarity by selectively causing the retraction of dendrites.

Keywords

leukemia inhibitory factor, ciliary neurotrophic factor, osteogenic protein-1, bone morphogenetic protein, dendritic retraction, dendrites, sympathetic neurons, stat3, gp130

DOI

10.1523/JNEUROSCI.19-06-02113

Volume

19

Issue

6

First Page

2113

Last Page

2121

Disciplines

Biology | Life Sciences

Original Citation

Guo X., Chandrasekaran V., Lein P.J., Kaplan P.L. and Higgins D.: Leukemia inhibitory factor and ciliary neurotrophic factor cause dendritic retraction in cultured sympathetic neurons. J. Neurosci., 19: 2113 - 2121, 1999. 10.1523/JNEUROSCI.19-06-02113.

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