Glia induce dendritic growth in cultures sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists
SMC Affiliated Work
1
Status
Faculty
School
School of Science
Department
Biology
Document Type
Article
Publication Date
12-1-2002
Publication / Conference / Sponsorship
J. Neurosci
Description/Abstract
Dendritic growth in cultured sympathetic neurons requires specific trophic interactions. Previous studies have demonstrated that either coculture with glia or exposure to recombinant bone morphogenetic proteins (BMPs) is both necessary and sufficient to induce dendrite formation. These observations led us to test the hypothesis that BMPs mediate glial-induced dendritic growth. In situ hybridization and immunocytochemical studies indicate that the spatiotemporal expression of BMP5, -6, and -7 in rat superior cervical ganglia (SCG) is consistent with their proposed role in dendritogenesis. In vitro, both SCG glia and neurons were found to express BMP mRNA and protein when grown in the presence or absence of the other cell type. However, addition of ganglionic glia to cultured sympathetic neurons causes a marked increase in BMP proteins coincident with a significant decrease in follistatin and noggin. Functional assays indicate that glial-induced dendritic growth is significantly reduced by BMP7 antibodies and completely inhibited by exogenous noggin and follistatin. These data suggest that glia influence the rapid perinatal expansion of the dendritic arbor in sympathetic neurons by increasing BMP activity via modulation of the balance between BMPs and their antagonists.
Volume
22
Issue
23
First Page
10377
Last Page
10387
Disciplines
Biology | Life Sciences
Rights
Open Access article
Original Citation
Lein P.J., Beck H., Chandrasekaran V., Gallaghar P.J., Chen H, Lin Y, Guo X., Kaplan P.L., Tiedge H. and Higgins D.: Glia induce dendritic growth in cultures sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists. J. Neurosci., 22: 10377-10387, 2002. https://www.ncbi.nlm.nih.gov/pubmed/12451137
Repository Citation
Chandrasekaran, Vidya and author(s), additional. Glia induce dendritic growth in cultures sympathetic neurons by modulating the balance between bone morphogenetic proteins (BMPs) and BMP antagonists (2002). J. Neurosci. 22 (23), 10377-10387. [article]. https://digitalcommons.stmarys-ca.edu/school-science-faculty-works/180
