SMC Author

Karen Ruff

Status

Faculty

School

School of Science

Department

Chemistry

Document Type

Article

Publication Date

9-22-2016

Publication / Conference / Sponsorship

RNA

Description/Abstract

The glycine riboswitch often occurs in a tandem architecture, with two ligand-binding domains (aptamers) followed by a single expression platform. Based on previous observations, we hypothesized that "singlet" versions of the glycine riboswitch, which contain only one aptamer domain, are able to bind glycine if appropriate structural contacts are maintained. An initial alignment of 17 putative singlet riboswitches indicated that the single consensus aptamer domain is flanked by a conserved peripheral stem-loop structure. These singlets were sorted into two subtypes based on whether the active aptamer domain precedes or follows the peripheral stem-loop, and an example of each subtype of singlet riboswitch was characterized biochemically. The singlets possess glycine-binding affinities comparable to those of previously published tandem examples, and the conserved peripheral domains form A-minor interactions with the single aptamer domain that are necessary for ligand-binding activity. Analysis of sequenced genomes identified a significant number of singlet glycine riboswitches. Based on these observations, we propose an expanded model for glycine riboswitch gene control that includes singlet and tandem architectures.

Keywords

aptamer, gene control, glycine, metabolite, riboswitch

Scholarly

yes

DOI

10.1261/rna.057935.116

Volume

22

Issue

11

First Page

1728

Last Page

1738

Disciplines

Chemistry

Rights

Open Access journal

Creative Commons License

Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Comments

https://doi.org/10.1261/rna.057935.116

Original Citation

Karen Ruff (Chemistry): “Singlet glycine riboswitches bind ligand as well as tandem riboswitches,” with Ayesha Muhammad, Phillip J. McCown, Ronald R. Breaker and Scott A. Strobel, in RNA, Published in Advance, September 22, 2016, doi:10.1261/rna.057935.116.

Download

Included in

Chemistry Commons

Share

COinS