Title

Preparation of Outer Membrane Vesicles from Myxococcus xanthus

Status

Faculty

School

School of Science

Department

Biology

Document Type

Article

Publication Date

1-20-2016

Publication Title

Bio-Protocol

Description/Abstract

Outer membrane vesicles (OMVs) represent a unique sub-cellular compartment of bacteria that may act as a scaffold for various extracellular activities, including intercellular signaling. Myxococcus xanthus (M. xanthus) is a predatory bacterium that engages in cell-cell behaviors such as fruiting body formation and contact dependent lysis of other microbes. The OMVs of M. xanthus have been shown to have an elaborate architecture of chains and tubes that can connect cells within a biofilm. These higher order OMV structures have been shown to contain proteins exchanged for community behaviors and small molecules that have antibiotic activities, and may help facilitate directed exchange. M. xanthus OMVs allow material transfer between neighboring cells for motility and predation.

Keywords

EPS, Biofilm, Cell fractioning, Proteomics, Extracellular

SMC Affiliated Work

1

Volume

6

Issue

2

First Page

e1716

Scholarly

yes

DOI

10.21769/BioProtoc.1716

Disciplines

Biology | Cell Biology | Microbiology

Original Citation

James Berleman (Biology): “Preparation of Outer Membrane Vesicles from Myxococcus xanthus,” by Berleman JE, Marcin Zemla, Jonathan P Remis and Manfred Auer, in Bio-Protocol 6(2): e1716; 2016 Jan 20; doi: 10.21769/BioProtoc.1716. http://www.bio-protocol.org/e1716.

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